非洲菊耐热变异离体筛选体系的研究

The establishment of the in vitro screening procedure of heat-tolerant variants of Gerbera hybrida

以非洲菊(Gerbera hybrida)6个品种为材料,通过未生根组培苗的耐高温实验,结合细胞膜相对电解质渗透率测定与增殖率统计,确定了45℃20h、35℃10～25d处理是各品种合适的热胁迫条件。高温处理后的组培苗细胞膜相对电解质渗透率与存活率之间存在良好的相关性,不同品种间的耐热能力有差异,同一品种在35℃与45℃下的耐热性表现不同。

For further screening of heat-tolerant variants six cultivars of Gerbera hybrida were used as materials to be cultured in vitro. The proper treatment times were determined under 35℃ and 45℃ by the measurement of relative electrolyte leakage of cell membrane and the calculation of proliferation rate. The results showed that the relative electrolyte leakage of cell membrane had good correlation with the survival rate of the shoots in vitro after the same heat treatment. There were differences among the heat-tolerance of all cultivars, as well as the same cultivars under 35℃ and 45℃. 20-hour treatment at 45℃ and 10～20-day treatment at 35℃ were the proper heat treatment for all cultivars in our experiments.