早期应用维生素E对急性胰腺炎大鼠胰腺细胞凋亡和胰腺组织病变的影响

The effect of Vitamin E on apoptosis and pancreatic lesions in rats with acute pancreatitis in early stage

目的 :探讨早期应用维生素E(Vit.E)对急性胰腺炎 (AP)大鼠胰腺细胞凋亡和胰腺组织病变的影响。方法 :将SD大鼠 72只随机分为对照组、AP组和Vit.E治疗组 ,每组 2 4只。经十二指肠行胆胰管逆行加压注射 4 %的牛磺胆酸钠 ,诱导大鼠AP模型。于术后 3h、6h、12h和 2 4h采取断颈方法分批处死动物 ,应用末端脱氧核苷酸转换酶 (TdT)介导的原位末端标记 (TUNEL)法检测胰腺细胞凋亡情况 ,观察各组胰腺组织的病理切片并进行组织病理学评分。结果 :AP组和Vit.E治疗组术后胰腺凋亡细胞明显增多 ,两组在 3h和 6h的凋亡指数均无明显差异 (P >0 .0 5 ) ,而Vit.E治疗组在 12h和 2 4h的凋亡指数显著高于对应时相的AP组 (12h ,P <0 .0 5 ;2 4h ,P <0 .0 1) ;与AP组比较 ,Vit.E治疗组胰腺组织病变较轻 ,12h和 2 4h的胰腺组织病理学评分明显低于AP组 (12h ,P <0 .0 5 ;2 4h ,P <0 .0 1)。结论 :早期应用抗氧化剂Vit.E能够有效地减轻AP胰腺病变的严重程度 ,而胰腺细胞凋亡可能是介导此过程的一个重要病理、生理改变。

Objective:To investigate the effect of Vitamin E(Vit.E) on apoptosis and pancreatic lesions in rats with acute pancreatitis in early stage.Methods:72 SD rats were randomly divided into 3 groups:acute pancreatitis(AP:n=24),Vit.E treatment(n=24) and control(n=24). The model of AP was induced by retrograde injection of 4% sodium taurocholate into biliopancreatic duct in rats. At 3 h,6 h,12 h and 24 h,groups of rats were separately sacrificed by cervical transection. A terminal deoxynucleotidyl transferase-mediated dUTP-biotion nick end labeling(TUNEL) was used to detect apoptosis. Pancreatic histopathological score based on microscopic changes was evaluated.Results:Apoptoic acinar cells increased in AP and Vit.E treatment rats. Apoptosis index(AI) of Vit.E treatment rats at 12 h and 24 h was higher significantly than that of AP rats(12 h,P<0.05;24 h,P<0.01,respectively). The pancreatic histopathologic damages in AP and Vit.E treatment rats become gradually severer after AP induction. The pancreatic histopathologic scores of Vit.E treatment rats at 12 h and 24 h,compared with that of AP group,were lower significantly(12 h,P<0.05;24 h,P<0.01,respectively).Conclusion:Administration of Vit.E might effectively alleviate the sevevity of AP,in which pancreatic acina cells undergoing apoptosis may be an impotant pathologic changes.