三种胚胎移植技术方法比较

Comparison of Embryo Transfer Methods of Laboratory Mice

以近交系FVB、DBA 2小鼠作为供体胚小鼠 ,封闭群CD - 1小鼠作为受体小鼠 ,对实验小鼠胚胎移植的三种方法进行比较 ,即 :对小鼠受精卵 1细胞期胚 (或 2细胞期胚 )经由受体小鼠输卵管口移植、经由受体小鼠输卵管壁移植及对小鼠进行子宫移植 (8细胞期胚 )的方法进行了实验研究 ,并对各方法适用于使用的动物对象、实验要求、产仔率、优势与不足等做了进一步探讨 ,为今后的实验动物提供参考依据。结果表明 ,输卵管壁移植优于输卵管伞口移植和子宫移植。

Three embryo transfer methods of laboratory mice: the oviduct transfer,the transfer through the wall of the fallopian tube and the uterine transfer were studied and compared by using the embryos from FVB and DBA/2 mouse,and CD-1 mouse as foster mother.It was also discussed for each transfer method that which is better to different mouse strains,different experiment,and different litter birth rates.The results may provide a useful reference material for some of laboratory animals and animal experiments.The result shows that the transfer through the wall of the fallopian tube is excel to the oviduct transfer and the uterine transfer.