犬瘟热病毒A株N蛋白基因编码区的克隆与序列分析

Cloning and Analysis of Gene Encoding the N Protein of Canine Distemper Virus A Strain

本试验对犬瘟热病毒 ( CDV) A株 N蛋白基因编码区进行了克隆与序列分析。结果表明 :CDV A株 N蛋白基因编码区核苷酸序列与Onderstepoor株。 A75/ 1 7株及 2 544/ han95株的同源性分别为 97.5%、94%及 93 % ,编码氨基酸的同源性分别为 98%、97%和 96%。

The sequences of gene encoding the N protein of Canine Distemper Virus(CDV) A strain was cloned into pMD18-T vector and analysed the sequences.The result showed that the N protein of CDV A strain compare with Onderstepoor strain,A75/17 strain and 2544/han95 strain,the sequence homology of nucleotide were 97.5%,94% and 93%,amino acids were 98%,97% and 96%,respectively.