头孢地秦和阿米卡星治疗肺炎克雷伯氏菌感染对小鼠胸腺细胞凋亡的影响

Effects of cefodizime and amikacin on thymocyte apoptosis in mice induced by Klebsiella pneumoniae infection

目的研究肺炎克雷伯氏菌感染及应用头孢地秦、阿米卡星治疗后对NIH小鼠胸腺细胞凋亡的影响。方法给NIH小鼠腹腔注射肺炎克雷伯氏菌悬液,建立肺炎克雷伯氏菌感染模型。TUNEL法染色后用流式细胞仪观察24h内胸腺细胞的凋亡量。腹腔注射不同剂量的头孢地秦和阿米卡星,观察注射后9h胸腺细胞的凋亡量。结果胸腺细胞凋亡在感染后逐渐增高,至24h达高峰(P<0.01)。与感染组相比,头孢地秦5、15mg组的凋亡率分别下降56%、81%,两组比较有显著性差异(P<0.05)。阿米卡星5、15mg组的凋亡率分别下降41%、76%,两组比较也有显著性差异(P<0.05)。结论肺炎克雷伯氏菌感染能引起胸腺细胞发生凋亡,应用头孢地秦和阿米卡星能明显抑制胸腺细胞的凋亡。

Objective To investigate the effects of Klebsiella pneumoniae infection and the treatments of cefodizime and amikacin on the apoptosis of thymocyte in NIH mice. Methods Sepsis model of mice was established successfully by i.p suspension of Klebsiella pneumoniae. Apoptosis in thymocytes induced by bacterial infection and effects different dosage of cefodizime(ip) and amikacin(ip) were detected with flow cytometric assessment by TUNEL staining. Results The apoptosis in thymocyte increased continuously and reached its peak in 24 hours after infection.The thymocyte apoptosis rate were decreased significantly after injection of 5,15mg cefodizime by 56%,81%,or 5,15mg amikacin by 41%,76%,compared with the infections (P< 0.05). Conclusion The apoptosis in thymocyte can be induced by Klebsiella pneumoniae infection and the treatment of cefodizime and amikacin can significantly inhibit the thymocyte apoptosis.