组织工程血管脱细胞支架主要组织相容性复合体Ⅰ抗原性的免疫组化观察

Immunohistochemical observation of major histocompatibility complex class Ⅰ ant igenicity in decellularized scaffolds of tissue engineered blood vessels

目的 :探讨组织工程血管全生物支架的免疫原性的检测方法。 方法 :取猪颈总动脉 ,剥离外膜 ,剪成 2 cm长的血管段 ,随机分为 3组 :对照组、脱细胞组和脱细胞加碱处理组 ,每组 5段。以免疫组化方法检测血管脱细胞处理前后主要组织相容性复合体 (MHC )的变化情况 ,并将检测结果作图像分析。 结果 :对照组血管壁 MHC 高表达 ,脱细胞处理后 MHC 明显减少。图像分析得出阳性颗粒面积与视场面积比 :对照组为 (2 4 .0 9± 6 .0 9) % ,脱细胞组为 (6 .71± 2 .1 8) % ,脱细胞加碱处理组为 (1 .6 2± 0 .76 ) % ,组间比较差别均有显著意义 (P<0 .0 1 )。 结论 :MHC

Objective:To detect the immunog enicity of the bioscaffold used in building tissue engineered blood vessels.Methods:The carotid arteries of pigs were cut into 2 cm long segme nts and were randomly divided into 3 groups:the control group,the decellulariz ed group,and the decellularized/base treated group.Major histocompatibility c omplex class Ⅰ(MHCⅠ),which was highly expressed in vascular mural cells,was ch osen t o evaluate the antigenic changes of the vessels before and after decellularized treatment.The staining results were analyzed with image analysis system.Results:MHCⅠ was highly expressed in the control group,while after treatments its detectable rate dropped significantly.The ratio of positive gra nular area to the visual area was (24.09±6.09)% in the control group,(6.71±2.1 8 )% in the decellularized group and (1.62±0.76)% in the decellularized/base tre ated group.The differences among groups were significant ( P <0.01).C onclusion:MHCⅠ immunohistochemical staining can serve as a valuable m ethod to detect the antigenicity of the vascular bioscaffold.