BrdU-ELISA法测定四逆汤及组方药提取物对大鼠血管平滑肌细胞增殖的影响

Effect of extracts from Sini Tang and its component drugs by BrdU-ELISA on proliferation of rat vascular smooth muscle cell in vitro

目的 观察四逆汤 (SNT)及组方药提取物对大鼠主动脉血管平滑肌细胞 (VSMC)增殖的影响。方法采用 MTT比色法和 Brd U- EL ISA法观察药物对离体大鼠 VSMC增殖的影响。结果 Brd U- EL ISA法结果表明四逆汤总提取物 (EST)对 VSMC的 Brd U掺入 DNA没有显著影响 ,而 MTT测定表明 EST可以提高 VSMC的脱氢酶活性。甘草 (L E)、附子 (AE)、干姜 (GE)、甘草加附子 (L AE)、干姜加附子 (AGE)提取物显著提高VSMC的脱氢酶活性 ,同时提高 VSMC的 Brd U掺入 DNA的水平。结论 EST可以增加 VSMC的脱氢酶活性 ,但对 VSMC的 Brd U掺入 DNA的水平无影响。而其他组方药提取物可以增加 VSMC的脱氢酶活性 ,同时提高VSMC的 Brd U掺入 DNA的水平。

Object To observe the effect of extracts from Decoction for Resuscitation (Sini Tang) (EST) and its component drugs on proliferation of rat vascular smooth muscle cells (VSMC). Methods Using methyl thiazolyl tetrazolium assay (MTT assay) and bromodeoxyuridine (BrdU) ELISA,to investigate the effect of drugs on proliferation of VSMC in vitro. Results The results indicated that EST increased dehydrogenase activity (MTT assay) but had no effect on BrdU incorporation in DNA in VSMC. Prepared licorice root extracts (LE),aconite root extracts (AE),dried ginger extracts (GE),extracts of prepared licorice root and aconite root (LAE),extracts of aconite root and dried ginger (AGE) increased dehydrogenase activity and BrdU incorporation in DNA in VSMC of rat. Conclusion EST increased dehydrogenase activity but had no effect on BrdU incorporation in DNA in VSMC. LE,AE,GE,LAE,AGE increased dehydrogenase activity and BrdU incorporation in DNA in VSMC of rats.