不同采收期溪黄草中2α-羟基熊果酸含量的动态研究

Determination of 2α-hydroxy-ursolic acid from Herba Rabdosiae Serrae in various collecting periods

目的 为确定溪黄草最佳采收期提供依据 ,为按中药材 GAP原则制定相关标准操作规程 (SOP)并实施推广应用 ,提供基础研究资料。方法 采用 RP- HPL C法测定 GAP基地产溪黄草药材不同采收期 2 α-羟基熊果酸的含量。色谱条件 :Kromasil RP- C1 8色谱柱 (2 5 0 m m× 4 .6 mm ,5μm ) ;乙腈 - 0 .0 5 %三氟乙酸溶液 (70 .5∶ 2 9.5 )为流动相 ;流速 0 .8m L/min;柱温 2 5℃ ;检测波长 2 10 nm。结果 不同采收期狭基线纹香茶菜药材中 2 α-羟基熊果酸的含量以 8月份为高 ,即开花前期。结论 建议狭基线纹香茶菜药材在开花前枝叶繁盛时采收。

Object To supply the basis for the optimal collecting period for Herba Rabdosiae Serrae (HRS). To lay down relevant Standard Operating practice (SOP) in accordance with GAP of Chinese medicinal materials and put them into practice with providing basic researching data. Methods To determine the contents of 2α-hydroxy-ursolic acid in HRS by various collecting periods (cultured under GAP) by RP-HPLC. Chromatographic conditions: Kromasil RP-C_ 18 (250 mm × 4.6 mm,5 μm) column;the mobile phase was acetonitrile-0.05% trifluoro-acetic acid solution (70.5∶29.5);the velocity of flow was 0.8 mL/min;column temperature was 25 ℃;and the detection wavelength was at 210 nm. Results In various collecting periods,contents of 2α-hydroxy-ursolic acid in HRS are the highest in the collection of earlier flower of Rabdosia Lophanthoides var. gerardianus (Beutham) H. Hara in August. Conclusion It suggests that HRS be gathered in the leaves luxuriance before blooming.