辛伐他汀对骨质疏松大鼠骨形成作用研究

An experimental study on the effect of simvastatin on bone formation in osteoporosis rats

目的 探讨他汀类药物对绝经后骨质疏松症骨形成的刺激作用和药理机制。方法 54只 SD雌鼠随机分为 3组 :去势后给药实验组 ,去势组和对照组 ,观察术后 4、1 0和 1 6 w骨形成指标 :骨钙素 (BGP) ,骨特异碱性磷酸酶 (骨 AKP)和总碱性磷酸酶 (总 AKP) ;骨吸收指标 :尿呲啶醚 (PYD)和脱氧呲啶醚 (DPD)。同步用 IBAS计算机全自动图像分析系统对不脱钙骨组织动态观测骨形态计量学参数。结果 给药实验组在去势后 4～ 1 6 w BGP和骨 AKP明显高于单纯去势组 (分别为 P<0 .0 0 1和 P<0 .0 5) ,而尿 PYD和 DPD两组间无显著差异 (P>0 .0 5)。骨形成表面(FS)和骨小梁体积 (TBV) ,骨小梁平均厚度 (MTT)较去势组明显升高 ,尤其是 TBV和 FS(分别为 P<0 .0 0 1和 P<0 .0 1 ) ,这种差异在第 1 0周最为显著。随着给药时间的延长 (第 1 6周 ) MAR逐渐增加 (P<0 .0 0 1 )。 RS与去势组比较在所有实验周均无差异。结论 辛伐他汀可刺激骨质疏松大鼠成骨细胞活跃增生 ,促进骨形成与骨转换速率 ,并可使骨质疏松症骨组织形态改善。

Objective To explore the stimulating effect and pharmacal mechanism of simvastatin on bone formation of postmenopausal osteoporosis .Methods 54 female SD rats were randomly divided into 3 groups: treatment with simvastatin group (G1) and ovariectomized group (G2) and control groups (G3). After 4 weeks?10 weeks and 16 weeks of operation, the bone formation parameters 〔bone osteocalin (BGP), serum bone specific alkaliphosphatase (bone AKP) and total alkaliphophatase (AKP)〕 and bone resorption indices 〔urine pyridinolin (PYD) and deoxypyridinoline (DPD)〕 were determined. At the same time, bone histomorphometry was measured with whole automobile imaging analysis system.Results 4-16 weeks after administration, BGP and bone AKP in GI increased significantly compared with G2 (respective P<0.001 and P<0.05), but the difference of PYD and DPD level in urine was not significant betweenGI and G (P>0.05). Trabecular bone volume (TBV) and formation surfase (FS) of G1 increased significantly than those of G2, this difference was most obvious at 10 week. The increase of MAR was in a time-depented manner.Conclusions The simvastatin could stimulate active hyperplasia of bone cell, promote bone formation and improve the shape of the bone of osteoporosis.