新生大鼠缺氧缺血后不同部位脑组织中Par-4基因的表达

The Diverse Expression of Prostate Apoptosis Response-4 Gene in Different Brain Regions of Hypoxic-Ischemic Neonatal Rats

目的:观察Par-4基因在新生大鼠脑缺氧缺血后,不同脑组织中的表达。方法:制备新生大鼠缺氧缺血性脑病的动物模型。利用RT-PCR检测脑缺氧缺血后不同时间点海马组织、大脑额叶皮质及小脑中Par-4的mRNA表达的改变,利用Westernblot检测在缺氧缺血24h后,海马组织大脑额叶皮质和小脑中Par-4蛋白表达量的改变。结果:①海马组织和大脑额叶皮质中的Par-4mRNA在脑缺氧缺血后2h已明显升高,至6h已达高峰。而小脑未见表达;②在新生大鼠脑缺氧缺血24h后,海马组织及大脑额叶皮质中Par-4蛋白表达量显著升高,并且海马组织中Par-4蛋白表达量高于大脑额叶皮质中Par-4蛋白表达量。而小脑未见其蛋白表达。结论:缺氧缺血对新生大鼠不同脑组织中Par-4基因表达影响不同,Par-4可能参予了缺氧缺血对新生大鼠海马组织和大脑额叶皮质的致病过程。

Objective: To investigate the various expression of prostate ap opto sis response-4(Par-4) gene in different brain regions of hypoxic-ischemic neo natal rats. Methods: The animal models of hypoxic-ischemic encephalopathy were prepared. At different time after attack of hypoxic-ischemia, the par-4 mRNA i n frontal lobes cortex, hippocampus, and cerebellum of neonatal rats was measure d by RT-PCR. Western blot was used to detect the expression of Par-4 protein a t 24 h after hypoxic-ischemia. Results: ①At 2 h after of hypoxic-ischemia, th e Par-4 mRNA in hippocampus and frontal lobes cortex was significantly elevated , and at its peak on 6 h. But it was not discovered in cerebellum; ②At 24h afte r attack of hypoxic-ischemica, the expression of Par-4 protein in hippocampus and frontal lobes cortex was significantly increased. And the levels of Par-4 p rotein in hippocampus was higher than that in frontal lobes cortes. But we could not find the Par-4 protein expression in cerebellum. Conclusion: Hypoxic-isch emia has diverse effect on different brain regions of neonatal rat for expressio n of Par-4 gene. Par-4 gene might be involved in the pathogenesis of hippocamp us′and frontal lobes′damage after attack of hypoxic-ischemia.