脑脊液中Tau蛋白及Aβ检测对老年期痴呆早期诊断的意义

Diagnostic Value of Tau and Aain Cerebrospinal Fluid in Senile Dementia

目的:探讨脑脊液(cerebrospinalfluid,CSF)中Tau蛋白及β-淀粉样蛋白(β-amyloidprotein,Aβ)对老年期痴呆(seniledementia,SD)主要包括老年性痴呆(Alzheimer'sdisease,AD)及脑血管病性痴呆(vasculardementia,VD)的诊断价值。方法:采用双抗体夹心酶联免疫吸附法(ELISA)检测54例AD患者,43例VD患者及30例正常对照者(normalcontrols,NC)脑脊液中Tau蛋白及Aβ1-42浓度。结果:老年期痴呆患者CSF中;①Tau蛋白浓度高于正常对照者(P<0.05);②Aβ1-42浓度低于正常对照者(P<0.05);③根据CSF中Tau蛋白、Aβ1-42浓度检测诊断老年期痴呆的灵敏度分别为51.55%、45.36%;特异性分别为90.90%、93.62%;结合Tau蛋白浓度及Aβ1-42浓度诊断SD的灵敏度为74.23%,特异性为93.33%。结论:临床上检测CSF中Tau蛋白、Aβ1-42浓度可作为早期诊断老年期痴呆的辅助指标。

Objective:To study the value of diagnosis on senile dementia i nclu ding Alzheimer's disease (AD) and vascular dementia(VD) by detecting Tau and a -amyloid protein(Aa) in senile dementia patients. Methods:The cerebrospinal f luid (CSF) levels of Tau and Aa1-42 in 54 patients with AD, 43 patients with V D and 30 normal controls (NC) were analyzed by sensitive enzyme-linked immunoso rbent assays (ELISA). Results:①The Tau levels in CSF of senile dementia are in creased compared with NC group. ②The Aa1-42 levels in CSF of senile dementia are decreased compared with NC group. ③By detecting the Tau concentrations and the Aa1-42 concentrations in CSF, the sensitivity of senile dementia is (51.55 %) and (45.36%) respectively, the specificity of senile dementia is (90.90%) and (93.62%) respectively; and the sensitivity and specificity of senile dement ia is 74.23%and 93.33%for combination of detection of Tau and Aa1-42 concent rations in CSF. Conclusion: The study suggests that the Tau levels and Aa1-42 in CSF are some potential diagnostic markers of senile dementia.