肠内免疫营养对烫伤大鼠免疫功能和炎症反应的影响

The effects of enteral immunonutrition on immunological function and inflammatory responses in severe scalded rats

目的 :研究肠内免疫营养物Stresson对烫伤大鼠免疫功能和炎症反应的影响及机制。 方法 :致 6 4只SD大鼠总体表面积 (TBSA) 30 %的三度烫伤 ,随机分为肠内免疫营养 (EIN)组及肠内营养 (EN)组。分别于伤前、伤后1、4、7、1 0天抽取静脉血 ,检测血清免疫球蛋白、T淋巴细胞亚群、血清内毒素 (LPS)、白细胞介素 6 (IL 6 )、肿瘤坏死因子α(TNF α)浓度。 结果 :烫伤后EN组CD3+ 、CD4 + CD8+ 、CD4 + /CD8+ 均显著降低 ,EIN组在伤后 7、1 0天CD3+ 、CD4 + 、CD4 + /CD8+ 比值与EN组相比显著升高 (P <0 .0 5或P <0 .0 1 )。烫伤后两组血清免疫球蛋白IgG、IgM、IgA的水平较伤前对照明显降低 ,但EIN组略高于EN组。EIN组和EN组血清内毒素、IL 6、TNF α的水平较伤前对照显著升高 (P <0 .0 1 ) ,EIN组血清内毒素、IL 6、TNF α的水平在伤后 4、7、1 0天较EN组明显降低(P <0 .0 5或P <0 .0 1 )。 结论 :早期EIN可以改善烫伤后免疫功能的低下 。

Objectives:To investigate the effects of enteral immunonutrition (Stresson) on immunological function and inflammatory responses in severe scalded rats. Methods:Sixty four SD rats inflicted by 30% TBSA Ⅲ degree scalds were randomly divided into enteral immunonutrition(EIN) and enteral nutrition (EN) groups.Animals of both groups were fed isocaloric enteral nutrition. Lymphocyte subsets, serum levels of immunoglobulins,endotoxin,IL 6,TNF α were determined on the 1st,4th,7th and 10th day postburn. Results:CD3 + ,CD4 + ,CD4 + /CD8 + in EIN group were higher( P <0.05 or P <0.01)than those in EN group on the 7th and 10th days postburn. The serum concentrations of IL 6, endotoxin and TNF α were singnificantly lower in EIN group than those in EN group( P <0.05 or P <0.01). Conclusions: Enteral immunonutrition could not only ameliorate the damage of immunological function, but also reduce inflammatory responses in serere scalded vaes.