17-β雌二醇对人粘液表皮样癌Mc3细胞增殖的影响

The effects of 17-β estradiol on the proliferation of human mucoepidermoid carcinoma Mc3 cells

目的 :研究 17-β雌二醇 (E2 )对体外培养的人粘液表皮样癌Mc3细胞增殖的调节作用。方法 :不同浓度、时间的E2 处理Mc3细胞 ,采用细胞计数、克隆形成测定、流式细胞术、免疫组织化学染色等方法 ,检测E2 对人粘液表皮样癌Mc3细胞系细胞群体倍增时间、克隆形成率、细胞周期分布以及对PCNA、Bcl -2阳性表达的影响。结果 :对照组E2 浓度为 10 -9、10 -8、10 -7mol/L时 ,其细胞群体倍增时间分别为 :3 6.0h、2 7.9h、2 8.8h、2 5 .7h ;细胞克隆形成率分别为 :13 .7%、18.1%、17.6%、2 0 .8% ;PCNA的阳性表达率分别为 :62 %、78%、74%、98% ;Bcl-2的阳性表达率分别为 :48%、82 %、77%、93 %。流式细胞术检查结果显示 10 -7mol/L的E2 处理 12、18、2 4h后 ,细胞周期中S期细胞所占比例分别为 :11.3 %、7.0 %、46.7%。结论 :一定浓度的雌激素具有促进细胞G1/S期转换 ,增加S期细胞数量 ,加速DNA合成 ,从而促进细胞增殖。

Objective:To investigate the effects of 17-βEstradiol(E 2)on the proliferation of human mucoepidermoid carcinoma Mc3 cells .Method:Mc3 cells were treated with various concentrations of E 2 for various time durations and then cell doubling time, colony-forming efficiency, cell cycle distribution were examined by cell counting, clonogenic assay and Flow cytometry; PCNA and Bcl-2 expression was studied by immunohistochemistry.Result:In the groups of control, 10-9,10-8 and 10-7mol/L E 2 treated the cell population doubling time(h) were 36.0,27.9,28.8 and 25.7; Colony-forming efficiency(%) were 13.7,18.1,17.6 and 20.8;PCNA expression (% of positive) 62,78,74 and 98; Bcl-2 expression (% of positive) 48,82,77and 93, respectively. When the cells had been treated with with E 2 at 10-7mol/L for 12h,18h and 24h, the proliferation index(PI) were 49.5, 27.5 and 33.6 respectively.Conclusion:E 2 may promote Mc3 proliferation at a certain rage of concentration.