持续性静压力对成骨样细胞MC3T3-E1生物学特性的影响

Effects of continuously compressive pressure on the osteoblast-like (MC3T3-E1) cells in vitro

目的 :研究持续性静压力对成骨样细胞MC3T3 -E1生物学特性的影响。方法 :利用已建立的细胞体外加力装置 ,给MC3T3 -E1细胞施加 2atm的持续性静压力 ,应用细胞计数 ,生化分析和放射免疫等方法 ,观察受力后不同时间细胞增殖、碱性磷酸酶活性以及骨钙素表达量等方面的变化。结果 :加力组细胞增殖速度减缓 ,实验组和对照组的细胞群体倍增时间分别为 72 .96h和 63 .3 6h ;随着培养时间的延长 ,细胞碱性磷酸酶活性增强 ,但加力组和对照组无明显差异 (P >0 .0 5 ) ;持续性静压力作用 2 4h后 ,与对照组相比 ,加力组骨钙素分泌的增加有显著差异 (P <0 .0 5 )。结论 :一定大小的持续性静压力在抑制MC3T3 -E1细胞生长的同时促进其分化成熟。

Objective:To determine the effect of continuously compressive pressure (CCP) on the biological characteristic of mouse ostoblast-like cells, MC3T3-E1, in vitro.Method:A self-made hydrostatic compressive device was used to apply 2atm CCP on the cells. The cellular proliferation was detected by cell counting method. Biochemical analysis and radio-immunological methods were used to study the effects of CCP on the alkaline phosphatase activity (ALP) and osteocalcin (OCN) synthesis at different intervals (8h, 16h, 24h, 48h). Result: The cells in CCP treatment group proliferated slower than those in control group, and the doubling time in the two groups are 72.96h and 63.36h respectively. Both of ALP activities and OCN synthesis in two groups increased with time, however, there was no significant difference of ALP activities between the two groups (p>0.05), and the secretion of OCN increased over 24h after applying CCP had a significant difference (p<0.05).Conclusion: CCP at 2atm may inhibit the growth of MC3T3-E1 cells and promote the differentiation of cells at the mean time.