T.Spelta 1Bs染色体育性基因Rf_3和rfk_1的分子标记

Molecular markers of the fertility genes Rf_3 and rfk_1 in the 1BS chromosome of T.Spelta

为了建立T.Spelta1Bs染色体育性基因Rf3和rfk1的分子标记辅助选育技术体系,提高选育效率,对T.Spelta1Bs染色体上育性基因Rf3和rfk1进行AFLP分子标记研究。结果表明,利用AFLP技术对小麦进行分子标记研究可获得50～100条清晰、稳定的带纹,多态性较高,重复性好。根据T型细胞质的育性,对TSP3314/绵阳26的F2群体采用集群分类的方法构建等基因池,利用AFLP技术筛选16个Pst /Taq 引物组合,然后对F2群体进行AFLP分析,获得2个引物组合P3-T2,P4-T3,其与T.Spelta1Bs染色体有关育性片段Rf3基因和rfk1基因连锁。然后结合TSP3314/绵阳26的F2群体在T型细胞质下的育性结果,和可育株与K3315A测交后代的育性分离所得的K型细胞质下的育性结果,运用Mapmaker软件进行分析,找到了与T.Spelta1Bs染色体育性基因Rf3和rfk1连锁的分子标记。

To breed non 1B/1R K type male sterile wheat lines,He Peiru designed a chromosome transfer method to transfer the fertility fragments in the 1Bs of T.Spelta (which including Rf_3 and rfk_1 genes) to common wheat,during this process,Rf_3 gene was used as the marker of the present of rfk_1 gene under the backgroud of T.timophvee cytoplasm.However,exchanges of the chromosome fragments were observed during the transformation progress,some materials were fertile male under the T.timophvee cytoplasm but did not carry the rfk_1 gene with them,it is necessary to study the molecular marker for the fertility fragments in the 1Bs of T.Spelta,which could be used in marker-assisted breeding of the non 1B/1R K type male sterile wheat lines.AFLP was used in the maker analysis of the fertility genes Rf_3 and rfk_1,which are located in the 1Bs chromosome of T.Spelta,the results showed that two primer combinations P3-T2 and P4-T3 could give 4 very good polymorphism bands respectively to the Rf_3 and rfk_1 genes based on the fertility of the F_2 individuals under both of the T.timophvee and Aegilops kotschyi cytoplasm.