银条不定芽诱导及高效增殖体系的建立

Establishment of adventitious bud induction and high efficient propagation system in many flower betony (Stachys floridana Schuttl.ex Benth.)

以"二细一粗"银条茎尖、叶片、茎段组织为外植体,研究了不同激素配比对其不定芽诱导与增殖效果的影响。结果表明,银条叶片组织未能再生;茎尖、茎段组织直接诱导较大不定芽的再生率较低,平均每个外植体最多可诱导不定芽5～7个。而通过茎段先诱导出不定芽芽团,再进行芽的伸长这一途径,每个外植体诱导的不定芽数平均可提高到14.3个,且芽团经5次继代后,增殖再生能力无衰退表现,且再生植株生产潜力非常巨大,是一条较优的再生途径。茎段不定芽芽团诱导培养基以添加6-BA5.0mg/L的MS培养基效果较好;增殖培养基以MS+6-BA4.5mg/L+GA31.0mg/L效果较好;不定芽伸长培养基以MS+6-BA1.5mg/L+NAA0.4mg/L+GA30.5mg/L较好。

The stem apex,leaf disc and stem segments as explants were used to study the effects of different hormones treatments on the adventitious bud induction and propagation of many flower Betony (Stachys floridana Schuttl.ex Benth).The results showed that stem apex and stem segments had low reproducing rate with only got 5-7 buds per explant in longer buds induction,while leaf disc had not showed ability to reproducing.The best way to get more reproducing plantlet (14.3 per explant)was to lengthen the bud from the bud lump which was gotten from the induced stem segments on MS medium supplemented with 6-BA 5.0 mg/L.The bud lump can be propagated for a long time and the reproducing ability would not decreased on MS medium with 6-BA 4.5 mg/L and GA_3 1.0 mg/L.It is founded the better medium for bud elongation is MS+6-BA 1.5 mg/L +NAA 0.4 mg/L +GA_3 0.5 mg/L.