葡萄幼胚胚状体发生与细胞学研究

Studies on Somatic Embryogenesis and Cell Morphology of Immature Grape Embryos

以葡萄未成熟胚为外植体诱导获得三类不同的愈伤组织,并对愈伤组织的细胞进行观察,发现细胞形态各不相同。只有颗粒状愈伤组织为胚性愈伤组织,其发生的胚状体与合子胚相似。

Immature zygotic embryos of six grape cultivars were cultured and observed. Three kinds of callus formed in Five cultivars. But the cell morphology of three kinds of callus were not the same. Only the calli like grain were embryogenic callus and exhibited embryogenic competence. The embryogenesis was similar to the zygotic embryo but had amount of abnormal embryos.