肝癌患者乙型肝炎病毒含量及C基因启动子基因变异的检测(英文)

Study on HBV-DNA concentration and HBV C Gene promoter mutation of hepatocarcinoma patients

目的 了解肝癌患者乙型肝炎病毒 (HBV)DNA含量及其与C基因启动子 (BCP)基因变异的关系。方法 采用PCR荧光实时定量技术检测 114例肝癌患者及 10 0例非肝癌乙肝患者HBVDNA含量。采用PCR -微板核酸分子杂交ELISA技术对肝癌及乙肝患者进行BCP基因变异检测。结果 肝癌患者 4 8%HBVDNA阳性 ,平均拷贝量为 4 .7× 10 6拷贝 /ml。BCP区域 176 2、176 4位突变率为 2 7% ,且BCP变异的患者HBV拷贝量明显大于非变异患者。 10 0例非肝癌乙肝患者HBVDNA阳性率 4 1% ,平均拷贝量 3.8× 10 5拷贝 /ml,BCP基因突变率为 8% ,肝癌患者的BCP基因突变率明显高于乙肝患者。结论 HBV感染可能是导致肝癌发生的重要原因 ,HBVBCP变异可能与病变程度有关。

Objective:To understand the relationship between HBV-DNA concentration and BCP gene mutation of hepatocarcinoma patients.Methods:FQ-PCR method was applied to detect HBV-DNA concentration in 114 hepatocarcinomas and 100 hepatitis B patients, PCR-microplate nucletide aid hybridization-ELISA technique was applied in the analysis of BCP gene mutation.Results:The HBV-DNA positive rate of hepatocarcinoma patients was 48%,the average HBV-DNA concentration was 4.7×10 6 copies/ml.The BCP gene mutation rate was 27%, HBV-DNA concentrations in BCP mutation samples were significantly higher than in non-mutation samples. The HBV-DNA positive rate of hepatitis B patients was 41%,the average HBV-DNA concentration was 3.8×10 5 copies/ml.The BCP gene mutation rate was 8%. The BCP gene mutation rate of hepatocarcinoma patients was remarkably higher than hepatitis B patients.Conclusions:HBV infection is an important factor leading to hepatocarcinoma and HBV BCP mutation is related to symptoms degree.