期刊文献+

生物乳化剂产生菌及其产乳化剂条件初步研究 被引量:14

BIOSYNTHESIS OF EMULSIEIER BY A NEWLY ISOLATED RHODOCOCCUS RUBER STRAIN Eml
下载PDF
导出
摘要 从胜利油田原油污染土壤边分离得到一株能降解多种石油芳烃和烷烃且能产生物乳化剂的细菌Em1,经生理生化及16S rRNA基因序列分析鉴定为赤红球菌(Rhodococcus ru-ber)。对菌株Em1产生乳化剂的条件进行了研究,结果表明,该菌株在以正十六烷为碳源时能较快速产乳化剂,其最佳条件为:正十六烷10g/L,酵母抽提物1g/L,初始pH值为7,30℃下200r/min摇床培养。在此条件下,发酵1d后,培养液的表面张力即降到最低值,约30mN/m,而乳化能力达100%;乳化剂浓度则在第5d达到最大,为68倍的CMC;经初步研究该乳化剂为脂类物质。 A bacterial strain Eml isolated from contaminated soil of Shengli oil field was identified as Rhodococcus ruber according to its phenotype, physiological and chemical properties, and its 16S rRNA gene sequence. This strain could degrade various polycyclic aromatic hydrocarbons as well as alkanes in petroleum, and produce bioemulsifier. The results indicated that strain Eml could produce bioemulsifier efficiently when n-hexadecane was used as sole carbon source. The optimal conditions for the synthesis of bioemulsifier were as followed: 10g/L of n-hexadecane, 1g/L of yeast extract, media initial pH 7, and cultivation was carried out at 30℃ on a rotary shaker at 200 rpm. Under these conditions the surface tension of culture decreased to the lowest value, around 30 mN/m, after 1 day, and the emulsifying capacity was 100% . The concentration of bioemulsifier reached to the highest value, around 68 times of CMC , after 5 days' cultivation. The results also showed that the bioemulsifier produced by this strain should be lipid.
出处 《微生物学通报》 CAS CSCD 北大核心 2003年第6期39-43,共5页 Microbiology China
基金 国家高技术研究发展计划项目("863"资助项目)(No.2002AA601150)
关键词 赤红球菌 生物乳化剂 石油污染 石油烃降解 Rhodococcus ruber, Bioemulsifler, Petroleum contamination, Degradation of hydrocarbons
  • 相关文献

参考文献3

  • 1张翠竹,张心平,梁凤来,贾莹,刘如林.一株地衣芽孢杆菌产生的生物表面活性剂[J].南开大学学报(自然科学版),2000,33(4):41-45. 被引量:30
  • 2李建武等合编.生物化学实验原理和方法[M].北京:北京大学出版社,1994..
  • 3北京大学化学系有机化学教研室编.有机化学试验[M].北京:北京大学出版社,1990..

二级参考文献4

  • 1王修垣 析玉 等.菌株H255原油发醇液乳化物质的分析[J].微生物学报,1982,22(3):269-275.
  • 2李建武,生物化学实验原理和方法,1994年,227页
  • 3Jain D K,J Microbiol Methods,1991年,3卷,271页
  • 4王修垣,微生物学报,1982年,22卷,3期,269页

共引文献29

同被引文献137

引证文献14

二级引证文献67

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部