海藻糖合成酶活性受固定化处理过程影响的研究

THE EFFECT OF IMMOBILIZATION PROCESS TO THE ACTIVITY OF MTHASE AND MTSASE

在麦芽糖苷基海藻糖合成酶(MTSase)和麦芽糖苷基海藻糖水解酶(MTHase)双酶的作用下,淀粉可转化为海藻糖,但是其转化率较低。文中采用多种固定化载体进行酶固定化研究,发现通过经戊二醛与壳聚糖交联后的载体与酶液作用,可吸附与海藻糖合成无关的杂酶和杂质,从而提高海藻糖合成酶的活性。通过比较固定化过程中与反应条件中多个因素的影响,得到了如下最佳作用条件:将酶液与经3％戊二醛交联18h后的滤纸作用18h,再与10％的淀粉溶液反应9h,与未经固定化作用比较,海藻糖的产率提高10倍,达到27.22g/L,转化率从5.33％提升到54.43％。

Amylum can be transformed into trehalose on the action of the MTSase and MTHase, however, the percentage of transformation is low. The research on the enzyme immobilization through four kinds of immobilization carrier is described in this paper. The result shows that the carriers, which are put into with enzyme liquid and crossbonded with chi-tosan and glutaraldehyde at first, can adsorb many enzymes independent from this process of trehalose synthesizing, consequently, the enzyme activity is increased sharply. By comparing with the influence of the immobilizing process and some factors in the reaction conditions, such optimize condition can be reached: the time for carrier crossbonding with glutaraldehyde is 18hours , the time with enzyme is another 18hours , and the time of reaction with amylum (10%) is 9hours.The result of the content of trehalose can reach 27.22g/L and the percentage of transformation can reach 54.43% , which previously is 2.67g/L and 5.33% respectively before dealt with by carriers.