鸡传染性支气管炎病毒结构蛋白基因的克隆及序列测定

Cloning and Sequencing of N Gene of Avian Infectious Bronchitis Virus T Strain

根据IBV病毒的已报道基因序列设计了用于扩增鸡传染性支气管炎病毒M41株S1基因和T株核衣壳蛋白基因(N)的引物,经RT-PCR得到了预期大小的扩增产物;将目的条带纯化并回收以后,克隆到pMD18-T质粒载体中,对插入片段进行序列测定,并与已发表的IBVS1、N基因序列进行了比较和分析,表明具有高度同源性。证明我们克隆了IBVS1和N结构蛋白基因。

A pair of primers was designed to amplify the N gene of IBV T strain based on the reported gene sequences. The anticipated product was acquired. After purification and withdraw , the PCR product was recombined into pMD18—T vector and sequenced. The sequence was compared with the reported N gene sequences and testified the high similarity to that of other IBV strains, which indicated the N gene of T strain had been cloned.