应用间接ELISA方法检测兔轮状病毒抗体的初步研究

Studies on Determination of Antibody of Lapine Rotavirus by Indirect ELISA

目的 建立间接ELISA方法对兔轮状病毒抗体进行检测。方法 确定抗原和阴、阳性参考血清的最佳工作浓度 ;进行特异性、敏感性以及中间试验。结果 抗原的最佳工作浓度为 1∶12 0 0 0倍 ;参考血清的最佳工作浓度为 1∶2 0 0倍 ;本次实验建立的ELISA方法具有很好的敏感性和特异性 ;中间实验表明 ,LaRV在兔群中具有很高的感染率。结论 本次试验成功地建立了用于兔轮状病毒抗体检测的ELISA方法。

Objective The aim of this study was to establish the method of ELISA for determination of antibody of lapine rotavirus.Methods The optimal concentration of antigen and the reference serum was decided in this study. The specific, sensitive and intermediate tests could be employed in this sdudy. Results The optimal concentration of antigen and the reference serum was 1∶12 000 and 1∶200. The sensitivity and specificity was very well in this method. Infectious rate of LaRV was very high in rabbits, demonstrated by the intermediate tests. Conclusion It was successful to establish the method of ELISA for determination of antibody of lapine rotavirus in this sdudy.