NSCLC中DNA-PKcs的表达及其与凋亡相关蛋白关系的研究

The expression of DNA-PKcs in non-small cell lung cancer and its relationship with apoptosis associated proteins

目的 检测非小细胞肺癌 (NSCLC)中DNA依赖蛋白激酶催化亚基 (DNA PKcs)的表达 ,探讨其与凋亡相关蛋白表达之间的关系。方法 采用免疫组化SP法检测 113例NSCLC患者肿瘤组织中DNA PKcs、p5 3和bcl 2的表达。 结果 NSCLC中DNA PKcs、p5 3和bcl 2的检出率分别为 89.3 8%、61.95 %及5 9.2 9% ;DNA PKcs的表达顺序依次为细支气管肺泡癌 >腺癌 >腺鳞癌 >鳞癌 ,而且DNA PKcs表达水平随肿瘤分化程度的增高亦逐渐增高 ,差异均具有高度显著性 (P <0 .0 5 ) ,但其表达与淋巴结转移无明显关系 ;p5 3在不同临床病理类型NSCLC中的表达无明显差别 ;bcl 2在不同组织学类型NSCLC中的表达顺序依次为鳞癌 >腺鳞癌 >腺癌 >细支气管肺泡癌 ,差异具有高度显著性 (P <0 .0 1) ,但其表达与肿瘤分化程度及淋巴结转移无关 ;DNA PKcs与 p5 3 (P <0 .0 1)、p5 3和bcl 2 (P <0 .0 5 )的表达之间有显著相关性。 结论 DNA PKcs高表达导致的DNA损伤修复系统活性增高以及突变型p5 3和bcl 2过表达导致的凋亡抑制相互影响、共同作用 。

Objective To study the expression of catalylic subunit of DNA dependent protein kinase (DNA PKcs) in non small cell lung cancer (NSCLC) and its relationship with apoptosis. Methods NSCLC tissues from 113 untreated patients were analyzed immnohistochemically with antibodies to DNA PKcs, p53 and bcl 2. Results There were expressions of DNA PKcs, p53 and bcl 2 in NSCLC at different levels. The positive rate of DNA PKcs, p53 and bcl 2 was 89.38%(101/113), 61.95%(70/113) and 59.29%(67/113) respectively. The expression of DNA PKcs was significantly related with the histological types. Its expression in squamous carcinoma was significantly lower than that in adenocarcinoma and bronchioloalveolar carcinoma. The expression of DNA PKcs increased with the increasing differentiated degree of NSCLC ( P <0.05), but had no relationship with lymph node metastasis. There was no significant relation between the expression of p53 and the pathological type of NSCLC. A significant difference of bcl 2 expression existed in the histological types of lung cancer ( P <0.01). Its expression in squamous carcinoma was significantly higher than that in adenocarcinoma, bronchioloalveolar carcinoma and adenosquamous carcinoma, but had no relationship with the differentiated degree of lung cancer and lymph node metastasis. The expressions were significantly related between DNA PKcs and p53 ( P <0.01), p53 and bcl 2 ( P <0.05). Conclusion The expression of DNA PKcs is fairly high in NSCLC. The high expression of DNA PKcs and overexpressions of mutated p53 and bcl 2 may be important causes of radioresistance in NSCLC.