大豆启动功能片段的PCR检测

AMPLIFICATION OF THE SOYBEAN PROMOTERBY POLYMERASE CHAIN REACTION

启动子在基因的表达调控中起着关键作用 .已克隆到大豆的启动子 ,并保存于质粒 pBI 1 0 1的多克隆位点 (HindⅢ和BamHI间 )上 .根据GenBank中质粒pBI 1 0 1、pBI 1 2 1的全序列设计引物 ,来扩增这一启动功能片段 .阴性、阳性对照都得到预期的泳带 ,但目的启动子带却与阴性带相同 ,说明质粒中的启动子片段在保存过程中已丢失 .

The promoter plays a key role in the gene expressing and regulating. Insert the soybean promoter cloned before into plasmid pBI 101 at the multiple clone sites (between restriction sites HindⅢ and BamHI). We design a pair of primers according to the complete sequence downloaded from the Genbank and amplify the target promoter. We get the predicted lanes from the positive and negative controls respectively, but not from the target plasmid, which might have been lost during conservation.