毛细管气相色谱法同时测定复方樟脑搽剂中樟脑、薄荷脑含量

Content determination of camphor and menthol in compound camphor liniment by capillary gas chromatography

目的:建立毛细管气相色谱法同时测定复方樟脑搽剂中樟脑、薄荷脑的含量。方法:采用气相色谱法,Agilent19095N-123INNOWAX毛细管色谱柱(30m×0.53 mm,1μm),FID检测器,柱温120℃,进样口温度220℃,检测器温度260℃,进样量1μl,分流比20∶1,载气为N2,柱流量6.0ml/min,空气流量450ml/min,H2流量40ml/min。结果:各组分的分离度和线性关系良好,樟脑、薄荷脑质量浓度线性范围分别为0.2016~2.0160mg/ml(r=0.9999)、0.3920~3.9200mg/ml(r=0.9999),平均回收率分别为99.01%(RSD=1.3%)、97.80%(RSD=1.8%)。结论:本法操作简便、结果准确、重现性好,可用于复方樟脑搽剂的质量控制。

Objective:To establish a method for the determination of camphor and menthol in compound camphor liniment by capillary gas chromatography(GC).Methods:The content was determined by GC combined with Agilent 19095N-123 INNOWAX capillary chromatographic column(30 m×0.53 mm,1μm).Column temperature:120℃,injector temperature:220℃,FID detector temperature:260℃.Injector volume was 1μl,split ratio was 20∶1.Nitrogen was used as carrier gas,the flow of column was 6.0ml/min,air:450ml/min,hydrogen:40ml/min.Results:The linear range were 0.2016-2.0160mg/ml(r=0.9999),0.3920-3.9200 mg/ml(r=0.9999)for camphor and menthol respectively.The corresponding average recovery rate were 99.01%(RSD=1.3%)and 97.80%(RSD=1.8%).Conclusion:This method is simple,accurate and with good reproducibility,which can be used as quality control for compound camphor liniment.