牡丹试管苗与扦插苗生根过程中相关酶活性的变化

Change in activities of related enzymes during rooting of peony shoots in vitro and cutting seedlings

【目的】研究牡丹品种‘凤丹白’无根试管苗和扦插苗生根过程中过氧化物酶(POD)、多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)活性的变化,探讨酶活性变化与牡丹生根的关系。【方法】以牡丹品种‘凤丹白’无根试管苗和扦插苗为材料,测定生根过程中POD、PPO和IAAO酶活性变化。【结果】在生根过程中,牡丹试管苗POD活性呈"下降-上升-下降-上升"趋势,PPO和IAAO活性均呈"上升-下降-上升"趋势。而扦插苗POD和PPO活性均呈"上升-下降-上升"趋势,IAAO活性呈"下降-上升-下降-上升"趋势。试管苗POD活性在第3天降到最低值,第1个峰值出现时间较扦插苗推迟1d;PPO活性第1个峰值较扦插苗提前2d,低值推迟1d;IAAO活性第1个峰值和低值出现时间均较扦插苗提前2d。【结论】与试管苗相比,牡丹扦插苗生根过程中POD、PPO及IAAO酶活性的极值出现时间均推迟,这可能与扦插苗生根时间较晚有关。

【Objective】The changes in POD,PPO and IAAO activities during rooting of shoots in vitro and cutting seedlings of Paeonia suffruticosa ‘Fengdanbai'were studied to explore the relationship of changes in enzyme activities and rooting of seedlings.【Method】Shoots in vitro and cutting seedlings of Paeonia suffruticosa variety‘Fengdanbai'were used as materials to measure the changes in POD,PPO and IAAO activities during rooting process.【Result】POD activity of shoots in vitro had a decrease-increase-decrease-increase trend while activities of PPO and IAAO had an increase-decrease-increase trend.POD and PPO activities of cutting seedlings showed a tendency of increase-decrease-increase,while IAAO activity had a trend of decrease-increase-decrease-increase.POD activity of shoots in vitro decreased to the minimum in the 3rd day,and the first peak was 1dlater than that of cutting seedlings.The first peak of PPO activity of shoots in vitro was 2dearlier than that of cutting seedlings,while the minimum was 1dlater.The first peak and minimum values of IAAO activity of shoots in vitro were all 2dearlier than those of cutting seedlings.【Conclusion】Compared with shoots in vitro,the extreme values of POD,PPO and IAAO activities of cutting seedlings appeared later,which could be related to the later roo-ting time of cutting seedlings.