不同剂量^(60)Co-γ辐照对朱砂叶螨的杀灭效果

Elimination of carmine spider mite(Tetranychus cinnabarinus(Boisduval)) by ^(60)Co-γirradiation with different dosages

【目的】研究不同剂量60 Co-γ辐照对朱砂叶螨(Tetranychus cinnabarinus(Boisduval))的杀灭效果,确定有效控制朱砂叶螨的60 Co-γ辐照剂量。【方法】分别用不同剂量60 Co-γ辐照处理朱砂叶螨卵(产后48h,处理剂量0,100,150,200,250Gy)、幼螨(0,100,200,300,400Gy)、前若螨(0,100,200,300,400Gy)和成螨(0,100,200,300,400,600,800Gy)后,将其置于(28±1)℃、相对温度60%~80%、光照16h/黑暗8h光周期下培养,每隔1d在20~80倍解剖镜下观察并记录朱砂叶螨生长发育、产卵、卵孵化及螨死亡的情况。【结果】朱砂叶螨48h卵经250Gy60 Co-γ辐照处理后均不能正常孵化,故250Gy的60 Co-γ对朱砂叶螨的卵具有明显致死效果;朱砂叶螨幼螨经300Gy剂量60 Co-γ处理后,发育成螨所产卵均不能正常孵化,表明300Gy及以上剂量处理朱砂叶螨幼螨可致其发育的成螨不育;400Gy的60 Co-γ可致朱砂叶螨前若螨发育的成螨不育;朱砂叶螨成螨经400,600和800Gy处理之后,分别在第19、17和15天达到100%死亡率,成螨在≥400Gy的辐照剂量下产卵量极显著减少,且所产卵均不能孵化。【结论】400Gy 60 Co-γ辐照处理能使各虫态朱砂叶螨致死或不育,可作为朱砂叶螨的有效检疫剂量。

【Objective】The experiment focused on the elimination of carmine spider mite(Tetranychus cinnabarinus(Boisduval))by 60Co-γirradiation to determine the effective irradiation dose for control of carmine spider mite.【Method】The eggs(48hafter delivery)of carmine spider mite were irradiated at doses of 0,100,150,200,and 250 Gy,the larvae and protonymphs of carmine spider mite were selected and irradiated at doses of 0,100,200,300,and 400 Gy,and adults were irradiated at doses of 0,100,200,300,400,600,and 800 Gy.After irradiation treatment,the mites were placed into the incubators at(28±1)℃,60%-80%relative humidity,and 16∶8(L∶D)light circulation.The growth and development,eggs laid,hatching rate and mortality were observed under a 20-80 times stereo microscope every other day.【Result】After being irradiated at the dose of 250 Gy,the 48h-old eggs of carmine spider mite could not hatch,indicating that 60Co-γgamma at the dose of 250 Gy had lethal effect on the eggs.Gamma irradiation at the dose of 300 Gy made the larvae sterile and the dose of the 400 Gy was sterile to the protonymphs of carmine spider mite.The eggs treated with doses of 400,600,and 800 Gy could not hatch.The results indicatedthat the dose of 400 Gy made carmine spider mite at different stages lethal or sterile.【Conclusion】60Co-γray at the dose of 400 Gy could be a generic quarantine treatment against potential infestation of carmine spider mites.