针对蛋白质转运的研究方法进展

The Progress of Methods Studying Protein Trafficking

蛋白质在核糖体被翻译出来后通过转运在细胞内的区室形成特殊定位和极化分布,这对于蛋白质发挥正常功能至关重要。新型标记技术和成像策略的出现能够直接观察到细胞内蛋白质的转运过程,以及用于研究转运调控的分子机制。该文着重于综述研究蛋白质转运的技术与方法策略。

Special localization and polarization distribution of protein which are achieved by trafficking after translation in ribosome is essential to maintain normal function of proteins.The emergence of novel labeling techniques and imaging strategies can directly be used to observe the transport process of proteins in cells and study molecular mechanism of protein trafficking.This review focuses on techniques and methodological strategies for studying protein trafficking.