ABCA1对滋养细胞基因表达谱的影响

Effect of ABCA1 on Gene Expression Profiles of Trophoblast Cells

该文从基因学角度探讨了ABCA1对人滋养细胞功能的影响。在人滋养细胞中调控ABCA1的表达,通过表达谱芯片检测ABCA1表达改变后滋养细胞中基因及相关信号通路的改变,Western blot及qRT-PCR验证芯片结果。结果显示,上调ABCA1表达时,有197个基因表达升高,有190个基因表达下降;而下调ABCA1表达时,有335个基因表达升高, 459个基因表达下降。GO和KEGG分析表明, ABCA1表达上升或下降后可导致滋养细胞内多个信号通路发生改变。qRT-PCR及Western blot检测发现,与阴性对照组相比, ABCA1上调后细胞中S1PR1的m RNA及蛋白水平明显升高,而CCL8、CXCL10与CXCL11的mRNA及蛋白水平明显降低;下调ABCA1的表达后,细胞中S1PR1的mRNA及蛋白水平明显降低,而CCL8、CXCL10与CXCL11的mRNA及蛋白水平明显升高,与芯片的结果完全一致。这些结果表明, ABCA1可通过调控多个信号通路而影响滋养细胞功能。

This artical explored the effect of ABCA1 on human trophoblast function from the genetic perspective.The expression of ABCA1 in human trophoblast cells was regulated,and the changes of genes and related signaling pathways in trophoblast cells were detected by expression microarray,and the results were verified by Western blot and qRT-PCR.The results showed that when ABCA1 expression was up-regulated,197 genes were up-regulated and 190 genes were down-regulated.When ABCA1 expression was down-regulated,335 genes were elevated in expression and 459 genes were down-regulated.GO and KEGG analysis indicate that increased or decreased ABCA1 expression leads to changes in multiple signaling pathways in trophoblasts.Compared with the negative control group,the mRNA and protein levels of S1 PR1 in the cells up-regulated by ABCA1 were significantly increased,while the mRNA and protein levels of CCL8,CXCL10 and CXCL11 were significantly decreased.The expression of ABCA1 was down-regulated after qRT-PCR and Western blot assay.The mRNA and protein levels of S1 PR1 in the cells were significantly decreased,while the mRNA and protein levels of CCL8,CXCL10 and CXCL11 were significantly increased,which was consistent with the results of the chip.These results indicate that ABCA1 can affect trophoblast function by regulating multiple signaling pathways.