人β防御素-2通过TGF-β/Smad信号通路对胃癌SGC7901细胞增殖、迁移和侵袭的影响

The Effect of Human β Defensin-2 on Proliferation, Migration, Invasion of Gastric Cancer SGC7901 Cells via TGF-β/Smad Signaling Pathway

该文探讨了人β防御素-2(hBD2)对胃癌SGC7901细胞的增殖、迁移和侵袭的影响。将真核表达载体pCMV-hBD2转染于人胃癌SGC7901细胞。采用qPCR和免疫印迹法(Western blot)检测转染效率。Western blot检测TGF-β1、p-Smad2/3、Smad2/3、MMP9的蛋白表达水平。Transwell法检测SGC7901细胞迁移和侵袭能力。EdU法和流式细胞术分别检测增殖能力与细胞周期。结果显示,转染真核表达载体pCMV-hBD2的SGC7901细胞, hBD2的表达水平明显高于SGC7901和转染pCMV-Blank的SGC7901细胞。SGC7901-hBD2细胞中TGF-β1、p-Smad2/3和MMP9的蛋白表达水平均低于SGC7901和SGC7901-Blank细胞,而Smad2/3表达水平不变。同时,其迁移侵袭和增殖能力均受到抑制,细胞周期G0/G1期阻滞。该实验结果表明, hBD2可能通过下调TGF-β/Smad信号通路调控SGC7901细胞的迁移侵袭以及增殖能力。

To study the effect of humanβdefensin-2(hBD2)on the proliferation,migration,invasion of gastric cancer SGC7901 cells,eukaryotic expression vector pCMV-hBD2 are transfected onto human gastric cancer SGC7901 cells.The expression of hBD2,TGF-β1,p-Smad2/3,Smad2/3 and MMP9 were detected by Western blot.Transwell assay was evaluated migration and invasion of SGC7901 cells.EdU method and flow cytometry respectively were detected cell proliferation and cell cycle.The results showed that overexpression of hBD2,the expression levels of hBD2 in SGC7901 cells transfected eukaryotic expression vector pCMV-hBD2 were higher than that in SGC7901 and SGC7901-Blank cells transfected pCMV-Blank.The expression levels of TGF-β1,p-Smad2/3 and MMP9 in SGC7901-hBD2 cells all decreased,while the expression levels of Smad2/3 remained unchanged.At the same time,its migration,invasion and proliferation were inhibited and cell cycle G0/G1 phase was blocked.It is possibly that hBD2 regulates the migration,invasion and proliferation cycle of SGC7901 cells through downregulating the TGF-β/Smad signaling pathway.