盘基网柄菌DJ-1蛋白的亚细胞定位研究

The Subcellular Localization of DJ-1 Protein in Dictyostelium discoideum

DJ-1基因的突变或缺失导致帕金森病相关症状,但其在帕金森病中的作用以及其亚细胞位置尚存在争议。盘基网柄菌是研究神经退化性疾病的模式生物,通过绿色荧光蛋白(GFP)标记DJ-1蛋白,利用荧光蛋白技术及DJ-1与GFP共定位技术在正常和氧化情况下研究其在盘基网柄菌的亚细胞定位,可以为探索DJ-1蛋白亚细胞位置与致病机制之间的联系奠定基础。研究结果表明,正常情况下盘基网柄菌DJ-1蛋白位于细胞质内,一旦受到氧化应激, DJ-1蛋白则转移至线粒体,这个亚细胞位置转移与DJ-1蛋白C117位点的氧化相关。该研究为探索DJ-1蛋白如何在氧化应激条件下完成对细胞的保护提供了实验依据。

The loss and mutations of DJ-1 were reported to cause the symptoms related to Parkinson’s disease(PD),but the role of DJ-1 in PD and its subcellular localization still remained controversial.Dictyostelium discoideum is one of the recognized models and can be used to study the neurodegenerative disease.DJ-1 protein in D.discoideum was labelled by green fluorescence protein(GFP)and its subcellular localization under basal and oxidative stressed conditions was observed using immunofluorescence and colocalization of DJ-1 and GFP in order to discover the possible links between DJ-1 subcellular localization and its mechanisms related to PD.The results showed that DJ-1 was predominantly cytosol under basal condition in D.discoideum,but translocated to the mitochondria with hydrogen peroxide.This translocation of DJ-1 could possibly be due to the oxidation of site C117,which provides a crucial clue in studying how DJ-1 protects the cells under oxidative stressed condition.