有机泡沫浸渍法制备多孔β-TCP支架及生物相容性研究

The Preparation of Porousβ-TCP Scaffold by Foam Impregnationand Its Biocompatibility

以制备满足骨组织工程要求的支架为目的,将β-TCP粉末与Al2O3-MgO-H3PO4粘结剂按一定比例调和成浆料,采用有机泡沫浸渍法使其成型,低温烘干后于900-1200℃烧结制得多孔β-TCP支架.然后接种从胚胎胫骨分离的成骨细胞,进行材料-细胞复合培养后,通过扫描电镜观察细胞在陶瓷表面附着、生长等情况,评价其生物相容性.研究表明,采用有机泡沫浸渍法制备的β-TCP支架具有三维开孔网状结构,而且复合培养后的陶瓷表面有许多形态良好的成骨细胞附着,具有良好的生物相容性.

The subject of this paper is to prepare β-TCP scaffold for bone tissue engineering. The β-TCP powders were mixed with appropriate amounts of Al2O3-MgO-H3PO4 based binder to form the slurry. The porous scaffold was produced using foam impregnation process. The green samples were dried at low temperature and sintered at 900-1200℃ so the scaffold gained. Osteocytes isolated from fetal shinbone were seeded on the β-TCP template to culture in vitro. The biocompatibility was evaluated through the observation of adhesion, growth of the osteocytes by SEM. The porous, which was made by foam impregnation process, possesses high porosity, open pore morphology. The surface of β-TCP scaffold was adhered by lots of osteocytes with perfect configuration when the scaffold-cell co-culture in vitro. These results indicate the porous β-TCP scaffold prepared by foam impregnation possesses good biocompatibility.