摘要
目的 探讨二氧化碳气体对结肠肿瘤细胞转移能力的影响。方法 体外培养人结肠癌CCL - 2 2 8细胞 ,应用二氧化碳或氮气处理不同时间后 ,应用免疫组织化学方法检测细胞中E钙粘蛋白 (E -Cadherin)和血管内皮生长因子 (VEGF)表达的变化。结果 二氧化碳处理 6 0min后CCL - 2 2 8细胞E -Cadherin表达显著降低 ,而VEGF表达显著增加。而氮气处理对上述指标产生相同影响但其作用明显弱于二氧化碳。结论 6 0min以上时间的二氧化碳气腹可以明显增强肿瘤细胞的转移能力 ,而氮气对其影响明显弱于二氧化碳 ,序贯应用二氧化碳、氮气、二氧化碳气腹的方法可改善二氧化碳气腹所致的影响。
Objective: To study the effects of carbon dioxide on the metastatic capability of colon cancer cells and compare them with nitrogen.Methods:The colon cancer cells CCL-228 were treated with 100% carbon dioxide or nitrogen for a series of time. 12 hours after the treatment, the expressions of E-cadherin and VEGF were examined. Results:After the treatment of carbon dioxide or nitrogen for over 60?min, the expression of E-cadherin decreased while the expression of VEGF was enhanced significantly. And the effects of nitrogen were similar to, but weaker than those of carbon dioxide.Conclusions:Carbon dioxide may improve the metastatic capability of cancer cells and the effects of carbon dioxide are significantly stronger than those of nitrogen. A sequential use of carbon dioxide and nitrogen in pneumopcritoneum may take the advantage of both gases.
出处
《中国现代医学杂志》
CAS
CSCD
2003年第24期18-21,共4页
China Journal of Modern Medicine
基金
SurportedbyNatureScienceFundofHubeiProvince( 2 0 0 0J0 62 )
