不同时辰电针大鼠“环跳”穴对血浆cAMP、cGMP水平的影响

EFFECTS OF ACUPUNCTURE ON PLASMA cAMP & cGMP LEVELS AT DIFFERENT TIMES OF THE DAY IN RATS

“子午流注”针灸疗法是中医颇具特色的治疗手段,临床实践表明疗效较好。用现代时辰生物学方法对这一独特疗法加以深入研究是有重要意义的。在许多细胞的生理活动中,cAMP,cGMP 是重要的第二信使,二者在细胞内的含量及其比例与细胞的功能状态有密切关系。已有文献报道人血浆 cAMP 水平存在近日节律性变化;

96 healthy male Wistar rats were housed in a light-controlled room forover 10 days prior to the experiment.Lights were on between 8:00 and 20:00each day.Food and water were ad arbilrium.The animals were divided into acupuncture group and control group.Experimental times were 5:00,11:00,17:00 and 23:00.After acupuncture at bilateral Huantiao points for 30 minutesthe animals were decapitated immediatly for plasma cAMP and cGMP radio-immunoassay.The results showed.1.The plasma level of cAMP presented adiurnal variation which peaked at 17:00 and (?)ouched the bottom at 23:00Acupuncture increases the concentrations of cAMP in all four times of the daybut did not alter its diurnal pattern.2.There was no significant differenceamong the plasma cGMP levels at the four limes of the day.Acupuncturecould increase the cGMP levels at 5:00,17:00 and 23:00 while not at 11:00,this suggests that the effects of acupuncture on plasma cGMP level are differentat different times of the day.