摘要
目的:比较不同血清学检测方法在梅毒诊断的应用特点。方法:2012年6月-2013年8月皮肤科门诊及住院患者18 360份标本,采用酶联免疫吸附试验(Tp-ELISA)、快速血浆反应素环卡实验(RPR)、梅毒螺旋体抗体明胶颗粒凝集试验(TPPA)进行检测,分析这3种检测手段在梅毒诊断中的应用特点。结果:TPPA法检测阳性413例,RPR的假阳性率9.74%(75/770),假阴性率25.45%(196/770),TP-ELISA的假阳性率8.44%(65/770),无假阴性率。结论:梅毒检测的各种方法各有其优缺点,必要时可将Tp-ELISA、RPR和TPPA联合检测以便减少临床误诊、漏诊的发生,从而减少不必要心理和经济负担。
Objective:To compare the application characteristics of different methods of serological detection in diagnosis of syphilis.Methods:18 360 specimens from department of dermatology and hospitalized patients were selected from June 2012 to August 2013.Those specimens were tested using enzyme linked immunosorbent assay(Tp-ELISA),rapid plasma reagin ring experiment(RPR) and treponema pallidum particle agglutination(TPPA),then analysed the characteristics of these three kinds of detection methods in diagnosis of syphilis.Results:413 cases were positive in TPPA test.The false positive rate of RPR test was9.74%(75/770).The false negative rate was 25.45%(196/770).The false positive rate of TP-ELISA test was 8.44%(65/770).The false negative rate was zero.Conclusion:Various methods of syphilis detection each has its advantages and disadvantages.Tp-ELISA,RPR and TPPA test can be combined if necessary,in order to reduce the occurrence of missed diagnosis and misdiagnosis,and to reduced unnecessary psychological and economic burden.
