胸腹水236例液基细胞学检测及免疫细胞化学分析

Liquid based cytology detection and immunocytochemistry analysis of 236 cases of hydrothorax and ascite

目的:探讨液基薄层细胞检测和传统涂片在胸腹水细胞病理诊断中的意义以及免疫细胞化学染色在恶性胸腹水诊断中的价值。方法:收集胸腹水标本236例,进行两种方法的制片,HE染色,并进行CEA、CK5/6、CK7、Ki-67免疫细胞化学染色。结果:液基细胞检测技术检测胸腹水恶性肿瘤检出率高于传统涂片技术,两组间差异有统计学意义(P<0.05);恶性胸腹水CEA、CK5/6、CK7、Ki-67表达阳性率高于良性胸腹水,两组间差异有统计学意义(P均<0.01)。结论:液基细胞检测技术检测胸腹水恶性肿瘤检出率高于传统涂片技术,辅以免疫细胞化学检测,对肿瘤的诊断、鉴别诊断及细胞来源有重要的价值。

Objective:To explore the significances of thinprep cytology test and conventional smea in the pathological diagnosis of hydrothorax and ascite cells,and the value of immunocytochemical staining in the diagnosis of malignant pleural effusion and ascites.Methods:236 cases of hydrothorax and ascite samples were selected.They were given two methods of production,HE staining,and CEA,CK5/6,CK7,Ki-67 immunocytochemical staining.Results:The detection rate of liquid based cell detection technology in the detection of hydrothorax and ascite malignant tumor was higher than that of conventional smear technique,and there was significant difference between two groups(P<0.05).The expression positive rate of CEA、CK5/6、CK7、Ki-67 in the malignant pleural effusion and ascites were higher than that of the benign hydrothorax and ascites,and there was significant difference between two groups(P<0.01).Conclusion:The detection rate of liquid based cell detection technology in the detection of hydrothorax and ascite malignant tumor was higher than that of conventional smear technique,supplemented by immunohistochemistry.It has important value on the tumor diagnosis,differential diagnosis and cell source.