肾脏病患者血清ANCA的检测与临床意义

Detection and clinical significance of serum ANCA in patients with kidney disease

目的:探讨抗中性粒细胞胞浆抗体(ANCA)的检测在肾脏病诊断中的临床意义。方法:应用间接免疫荧光(ⅡF)法检测抗ANCA,联合ELISA方法检测过氧化物酶(MPO)和蛋白酶3(PR3),观察99例肾脏病患者血清抗中性粒细胞胞浆抗体的阳性率。结果:应用ⅡF检测ANCA,联合ELISA检测MPO和PR3,肾脏病ANCA阳性率37%,其中肾小球肾炎、紫癜性肾炎、狼疮性肾炎阳性率分别为57%、21%和20%。结论:ANCA在肾小球肾炎和紫癜性肾炎中阳性率高,有重要诊断意义。

Objective:To investigate the clinical significance of anti neutrophil cytoplasmic antibody(ANCA) detection in renal diseases.Methods:We used the indirect immunofluorescence(ⅡF) assay to detect the anti neutrophil cytoplasmic antibodies,and combined with ELISA method for the detection of myeloperoxidase(MPO) and proteinase 3(PR3),and we observed the positive rate of serum anti neutrophil cytoplasmic antibody in 99 patients with kidney disease.Results:We used the ⅡF to detect the ANCA,and combined the ELISA to detect MPO and PR3.The positive rate of ANCA in kidney disease was 37%,and glomerulonephritis,purpura nephritis,lupus nephritis positive rates were 57%,21% and 20% respectively.Conclusion:The positive rate of ANCA in glomerular nephritis and purpuric nephritis is high,and there is important significance in diagnosis.