CD56^(bright)CD16^-NK细胞CD49d、CD11b及孕激素在稽留流产患者外周血的表达及意义

Expressions and significance of CD49d and CD11b on the CD56^(bright)CD16^-NK cell of early pregnancy

目的:探讨稽留流产患者外周血CD56brightCD16-NK细胞中整合素α4(CD49d)和整合素αm(CD11b)及血清孕酮(P)的表达及意义。方法:采集50例早期正常妊娠及50例稽留流产孕妇外周血,流式细胞术检测外周血CD56brightCD16-NK细胞含量及CD56brightCD16-NK中CD49d、CD11b表达,酶联免疫吸附法检测血清P含量。结果:稽留流产组外周血CD56brightCD16-NK细胞的表达较早期正常妊娠组显著降低(P<0.05);早期正常妊娠组的外周血CD56brightCD16-NK细胞上CD49d、CD11b的阳性表达率及血清P值均显著高于稽留流产组(P<0.05)。稽留流产组患者的血清CD49、CD11b和孕激素水平有一定的相关性(r=0.346,P<0.05)。结论:CD49d、CD11b及P可能在妊娠早期子宫自然杀伤(uNK)细胞的募集中起重要作用,同时也可能参与了正常妊娠的维持。

Objective: To investigate expressions and significance of integrinα4( CD49d) 、integrin αm( CD11b) on the CD56brightCD16-NK cell and serum progesterone( P) of the peripheral blood in missed abortion women. Methods: We collected peripheral blood of 50 cases normal early pregnancy women and 50 cases missed abortion. Flow cytometry was used to detect the peripheral blood CD56brightCD16-NK cells and the content of CD49d,CD11b in CD56brightCD16-NK. Serum P was detected by ELISA. Results: Expressions of CD56brightCD16-NK cell of peripheral blood in early pregnant women were higher than those in missed abortion women( P < 0. 05). Expressions of CD49d and CD11b on the CD56brightCD16-NK cell of peripheral blood in early pregnant women were higher than those in missed abortion women( P < 0. 05). Expressions of serum P of peripheral blood in early pregnant women were higher than that in missed abortion women( P < 0. 05). There was some correlation between missed abortion of pregnant women serum CD49,CD11b and progesterone levels( r = 0. 346,P < 0. 05). Conclusion: CD49d,CD11b and serum P may play an important role in the recruitment of uNK cells and participates in the maintenance of normal pregnancy.