摘要
目的:研究PTEN、PAX-2和β-catenin在正常子宫内膜、子宫内膜癌及癌前病变中的表达情况,探讨其与子宫内膜癌变的关系及作为临床诊断依据的可能性。方法:应用免疫组化法检测22例增生期子宫内膜、82例子宫内膜增生症和106例子宫内膜癌中PTEN、PAX-2和β-catenin的表达。结果:增生期子宫内膜、子宫内膜增生症、Ⅰ型子宫内膜癌中PTEN和PAX-2的阳性表达率逐渐递减。PTEN和PAX-2的阳性表达与子宫内膜病变的组织学类型及病变等级均呈负相关(相关系数tau-b=-0.513,P<0.05;tau-b=-0.609,P<0.05)。β-catenin在不同子宫内膜病变中的阳性表达率无显著差异(P>0.05),但在子宫内膜癌中β-catenin出现细胞核等异常表达。结论:PTEN、PAX-2的表达缺失和β-catenin的异常表达均与Ⅰ型子宫内膜样腺癌的发生相关,是Ⅰ型子宫内膜样腺癌的早期事件。PTEN、PAX-2对Ⅰ型子宫内膜样腺癌的早期诊断具有一定辅助意义,而β-catenin作为分子标记物的意义有待进一步研究。
Objective: To investigate the expression of PTEN,PAX-2 and β-catenin in endometrial carcinoma and precancers,and to discuss the possibility of them as useful biomarkers in clinical diagnosis. Methods: The expressions of PTEN,PAX-2 and β-catenin were detected by immunohistochemical staining in 22 cases of normal proliferative endometrium,82 cases of endometrial hyperplasia and 106 endometrial carcinoma. Results: The positive expression of PTEN and PAX-2 were gradually decreased in normal proliferative endometrium,typeⅠendometrial hyperplasia and endometrial adenocarcinoma( correlation coefficient tau-b =- 0. 513,P < 0. 05; tau-b =- 0. 609,P < 0. 05). The nucleus expression of β-catenin in different endometrium types was not significant( P > 0. 05). Conclusions: PTEN,PAX-2 lossing and β-catenin abnormal expression are all related to the occurance of typeⅠendometrial adenocarcinoma. They are the early events of typeⅠendometrial carcinogenesis. There are significant difference in the expression of PTEN and PAX-2 among normal proliferative endometrium,endometrial hyperplasia and type Ⅰ endometrial adenocarcInoma( except for simple hyperplasia and complex hyperplasia). PTEN and PAX-2,rather than β-catenin are useful biomarkers for early detection.
出处
《现代妇产科进展》
CSCD
2014年第2期85-88,共4页
Progress in Obstetrics and Gynecology
