人正常宫颈上皮细胞的体外分离及细胞活性比较

Normal human cervical epithelial cells in vitro comparative isolation and cell activity

目的:在传统上皮细胞消化分离方法基础上加以比较,探求一种最佳宫颈上皮细胞的体外分离方法。方法:取10例新鲜的正常宫颈组织,将同一组织分成大小相同的3部分,采用3种不同的消化方法(胰蛋白酶消化法、Ⅰ型胶原酶消化法、Ⅱ型中性蛋白酶+胰蛋白酶联合消化法)获取正常宫颈上皮角质细胞悬液,采用罗丹明B(SRB)法测定其细胞活性,同时比较其贴壁率。结果:胰蛋白酶组、Ⅰ型胶原酶组、Ⅱ型中性蛋白酶+胰蛋白酶联合消化组的细胞活性分别为0.9335、0.9544、0.9438,两两比较差异显著(P<0.01);Ⅰ型胶原酶消化组的细胞贴壁率显著高于其他两组(P<0.01)。结论:Ⅰ型胶原酶消化法是一种最理想的分离宫颈上皮细胞的方法。

Objective: An in vitro method for exploring the best of cervical epithelial cells.Methods: Take 10 cases of fresh normal cervical tissue,the same size,the same organization is divided into three parts,using three different digestion methods( trypsin digestion,Ⅰ collagenase digestion,Ⅱ-type neutral protease + trypsin digestion method combined) for normal cervical epithelial keratinocytes suspension,using rhodamineB( SRB) Law on its cell activity were measured,while comparing its adherence rate. Result: Trypsin group,Ⅰ collagenase group,Ⅱ-type neutral protease + trypsin digestion combined group were 0.9335,0.9544,0.9438 cell activity,the difference was highly statistically significant( P <0.01). Ⅰ collagenase group adherent cells were higher than the other two groups,the difference was statistically significant( P<0.01).Conclusions: Type Ⅰ collagenase digestion method is an ideal method of cervical epithelial cells isolated.