两次取精在夫精宫腔内人工授精中的应用

Applications of double semen collection following intrauterine insemination with husband's sperm

目的:探讨2次取精在夫精宫腔内人工授精(IUI)的临床应用价值。方法:回顾分析2008年1月~2012年8月316个夫精IUI周期,分析2次取精的各项精液指标及妊娠率;按注入宫腔内的前向运动精子总数(IMC)分为Ⅰ组(IMC>10×106个,281个周期)和Ⅱ组(IMC<10×106个,35个周期),比较2组的妊娠率。结果:两次取精的精液量、精子密度差异显著(P<0.01),而a级、b级精子比率无显著差异(P>0.05)。二次取精获得的IMC[(74.84±4.497)×106]显著高于一次[(48.60±3.105)×106](P<0.01);二次的妊娠率高于一次,但无显著差异(25.4%vs 16.7%,P>0.05)。Ⅰ组的妊娠率显著高于Ⅱ组(19.57%vs 8.57%,P<0.05)。结论:两次取精在夫精IUI中有实际应用价值。

Objective: To evaluate the clinical value of double semen collection in intrauterine insemination.Methods: A retrospective review of 316 cycles treated with intrauterine insemination with husband's sperm from Jan.2008 to Aug.2012.The several indicators and pregnancy rates of two semen collections were analyzed statistically.The cycles were divided into two groups according to the inseminating motile sperm count( IMC),group I which the IMC was higher than 10×106containing 281 cycles,however,59 cycles was included in group II which the IMC was less than 10×106,then the pregnancy rate was compared between them.Results: The amount of semen and sperm density in double semen collection showed significant difference( P<0.01),while there was no significant difference of sperm percentage between grade a and b( P>0.05).A significant increase of IMC[( 74.84±4.497) ×106]was seen in double semen collection compared with the first time [( 48.60±3.105) ×106]( P<0.01).The pregnancy rate in double semen collection was higher than the first time,but it showed no significance( 25.4% vs 16.7%,P>0.05).The pregnancy rate of group I was higher than group II and had significant difference( 19.57% vs 8.57%,P<0.05).Conclusion: There is clinical value of double semen collection in intrauterine insemination with husband's sperm.