玫瑰精B与赖诺普利相互作用及其荧光光谱分析与应用

Interaction between rhodamine B and lisinopril and their fluorescence spectrum analysis and application

探讨了玫瑰精B与赖诺普利的相互作用,建立了测定药物中赖诺普利的荧光分析新方法。在λex/λem=556 nm/585 nm、pH 7. 50 Tris-盐酸条件下,玫瑰精B与赖诺普利反应生成二元离子缔合物,使体系的荧光强度显著增强,赖诺普利的质量浓度在0. 06～4. 1 mg/L范围内与体系的荧光增强强度(ΔF)呈线性关系,检出限(3Sb/S)为0. 009 2 mg/L。该方法可用于市售赖诺普利片剂和胶囊中赖诺普利的测定,回收率为98. 7%～103%,相对标准偏差RSD(n=6)为2. 2%～2. 8%。

The interaction action between rhodamine B and lisinopril is discussed and a novel fluorescence analysis method for determination of lisinopril in drugs is established.Under the conditions of λex/λem= 556 nm/585 nm and Trishydrochloric acid of p H 7. 50,rhodamine B reacts with lisinopril to form a binary ion association complexes,which enhances distinctly the fluorescence intensity of the system.The mass concentration of lisinopril in the range of 0. 06 to4. 1 mg·L-1 is directly proportional to fluorescence enhancement intensity( ΔF) of the system,with a detection limit( 3 Sb/S) of 0. 009 2 mg·L-1. This method can be applied to the determination of lisinopril in commercially available lisinopril tablets and capsules,with recovery between 98. 7% and 103%,and the relative standard deviations RSD( n =6) in the range of 2. 2%-2. 8%.