小建中汤抗大鼠疲劳的作用机制初探

Preliminary study on the mechanism of Xiaojianzhong Decoction against rat fatigue

目的研究小建中汤抗疲劳及对免疫的调节作用。方法将60只SD大鼠随机分为空白组、模型组、小建中汤低剂量组、小建中汤中剂量组和小建中汤高剂量组,每组12只,雌雄各半。空白组和模型组大鼠每天给予蒸馏水1 mL灌胃,小建中汤低、中、高剂量组大鼠每天给予浓缩至600 mL/剂、400 mL/剂和200 mL/剂的小建中汤1 mL灌胃,连续灌胃15 d后进行跑步耐受测验,取血检测各组大鼠相关免疫因子白细胞介素-1(IL-1)、IL-6、补体蛋白3(C_3)、C_4、免疫球蛋白A(IgA)、IgG和IgM水平。结果小建中汤各组大鼠的跑步时间和路程均明显长于模型组(P均<0.05),并且随着小建中汤剂量的增加,大鼠跑步时间相应延长。模型组血清IL-1、IL-6水平均明显高于空白组(P均<0.05),血清C_3、C_4、IgA、IgG、IgM水平均明显低于空白组(P均<0.05)。小建中汤各组血清IL-1水平和小建中汤中、高剂量组血清IL-6水平均明显低于模型组(P均<0.05),小建中汤中、高剂量组血清C_3、C_4、IgA水平和小建中汤各组血清IgG水平、小建中汤高剂量组血清IgM水平均明显高于模型组(P均<0.05)。结论小建中汤具有抗疲劳的作用,调节免疫功能可能是其抗疲劳的作用机制之一。

Objective It is to study the anti-fatigue and immune regulation of Xiaojianzhong Decoction. Methods Sixty SD rats were randomly divided into blank group, model group, low-dose Xiaojianzhong Decoction group, medium-dose Xiaojianzhong Decoction group and high-dose Xiaojianzhong Decoction group, 12 rats in each group, half male and half female. The rats in the blank group and model group were intragastrically administered with 1 mL of distilled water per day. The rats in the low, medium and high dose groups of Xiaojianzhong Decoction were given a daily concentration of 600 mL/dose, 400 mL/dose and 200 mL/dose 1 mL of Xiaojianzhong. After treatment for 15 days, running tolerance test was performed, blood samples were taken to detect the levels of related immune factors interleukin-1(IL-1), IL-6 and complement protein 3(C3), C4, immunoglobulin A(IgA), IgG and IgM. Results The running time and distance of the rats in Xiaojianzhong Decoction groups were significantly longer than those in the model group(P<0.05), and the running time of the rats was prolonged with the increase of the dose of Xiaojianzhong Decoction. The levels of serum IL-1 and IL-6 in the model group were significantly higher while the serum levels of C3, C4, IgA, IgG and IgM were significantly lower than those in the blank group(P<0.05). The levels of serum IL-1 in Xiaojianzhong Decoction groups and serum IL-6 levels in medium and high dose Xiaojianzhong Decoction groups were significantly lower than those in the model group(P<0.05). The levels of serum C3, C4, IgA in the medium and high dose groups, IgG in every Xiaojianzhong groups and IgMl in the high-dose group were significantly higher than those in the model group(P<0.05). Conclusion Xiaojianzhongtang has anti-fatigue effect, and regulating immunity may be its anti-fatigue mechanism.