功能食品中违禁磷酸二酯酶5抑制剂类和减肥类西药的检测

Detection for PDE-5 Inhibitors and Weight Loss Drugs in Health Food

采用QuEChERS前处理技术提取、纯化功能保健食品中西地那非等磷酸二酯酶5(PDE-5)抑制剂类和酚酞等减肥类违禁西药化合物;TOF-MS高分辨质谱鉴定化合物准确分子量,定性分析86种PDE-5抑制剂类和66种减肥类药物,LC-Q-TOF-MS定量分析19种PDE-5抑制剂类和减肥类药物。14种PDE-5抑制剂类药物检出限为1.2-3μg/L,线性范围10-400μg/L;5种减肥类药物检出限为0.01-0.3μg/L,线性范围1-200μg/L。结果表明,该分析方法快速、灵敏、准确鉴定与测定功能保健食品中PDE-5抑制剂类和减肥类违禁药物。

It was studied that PDE-5 inhibitors and weight loss drugs in health food had been carried out. PDE-5 inhibitors and weight loss drugs were extraction and purified by Qu ECh ERS. With identification for 86 PDE-5 inhibitors and 66 weight loss drugs by high resolution TOF-MS, it was detected for 19 PDE-5 inhibitors and weight loss drugs by LC-Q-TOF-MS. The limits of detection about14 PDE-5 inhibitors were 1.2-3 μg/L and linear range 10-400 μg/L; in contrast, 5 weight loss drugs were0.01-0.3 μg/L and linear range 1-200 μg/L. The result showed that this method was rapid, sensitive and accurate, which was suitable for detection and identification of PDE-5 inhibitors and weight loss drugs in health food.