蝉花对造血功能的影响及其急性毒性实验研究

Effect of Cordyceps cicadae on the Hematopoietic Function and Its Acute Toxicity Test

目的观察蝉花对造血功能的影响和急性中毒情况。方法采用尾尖端放血和腹腔注射盐酸苯肼的方法,形成小鼠失血性贫血和盐酸苯肼贫血的模型,分组给药后采血测定小鼠的Hb、RBC,观察蝉花对造血作用影响。急性毒性试验为测定小鼠最大给药量方法。结果蝉花给药组与给水模型对照组比较有非常显著的差异。且高剂量组的作用与阿胶组相似。蝉花最大耐药量为80g/kg,相当于人用临床量444倍。结论证明蝉花具有明显抗失血性贫血和抗盐酸苯肼贫血作用。安全无毒。

OBJECTIVE To observe the effect of Cordyceps cicadae on the hematopoietic function and its acute toxic reaction.METHOD Two kinds of anemia model were established by losing blood from mice'tail top and injecting phenyl hydrazine hydrochloride,then Hb and RBC in the blood of mice were determined after being given Cordyceps cicadae in order to study its effect on the hematopoietic function.The maximal tolerable dose(MTD) of Cordyceps cicadae to mice was determined by acute toxicity test.RESULTS There had been significant difference between the experimental group given Cordyceps cicadae and the control group given water.Moreover,high dose Cordyceps cicadae had the similar effect on the hematopoietic function with donkey-hide gelatin.The MTD of Cordyceps cicadae was 80g/kg,which was 444 times of clinical daily dosage.CONCLUSION It showed Cordyceps cicadae had significant anti-action to hemorrhagic anemia and anemia caused by being injected phenyl hydrazine hydrochloride and that it was safe and un-poisonous.