柱切换HPLC法研究活性炭用量对阿霉素吸附程度的影响

Study on the effect of degree of adsorbing adriamicin according as dosage of activate carbonin by column switching HPLC

目的以活性炭作为吸附剂,确定阿霉素在血液灌流法治疗肝癌时所用吸附剂的最佳用量,以减少阿霉素的不良反应。方法采用含阿霉素的血浆灌注于不同组合量的活性炭柱,用柱切换高效液相色谱法测定阿霉素的灌注前后血药浓度。结果A组(150g×1支)、B组(150g×2支)、C组(150g×3支)、D组(150g×4支)及E组(150g×5支)活性炭柱对阿霉素的吸附率分别为20.19%、27.60%、55.98%、56.42%、57.13%,经方差分析有极显著性差异,经两两比较的q检验,C、D及E组间无显著性差异。结论除D组与E组P>O.05外,其它三个低剂量活性炭组中任意两组皆为P<0.01,故150g×3支活性炭柱为活性炭吸附剂的最佳用量,该用量的确定为血液灌流法治疗肝癌提供了理论依据。

OBJECTIVE To make sure the best dosage of sorbent which adsorbed Adriamicin(ADM) by activated carbon in hemoperfusion therapy the liver cancer,in order to reduce side effect of ADM.METHOD The blood plasma of ADM was perfusioned in the activated carbon columns which contanted the different combination.The blood concentration of ADM was determined by column switching HPLC.RESULTS The adsorption ratio of ADM by A group(150g × 1),B group(150g ×2) 、C group(150g ×3),D group(150g ×4)及 E group(150g × 5) activated carbon columns were 20.19%,27.60%,55.98%,56.42%,57.13%.The mean difference is significant at the 0.01 level by ANOVA.It was no difference significant at the 0.05 leve between groups of C,D and E by post hoc multiple comparisons.CONCLUSION There were difference significant at the 0.01 leve between any two of three groups of low dosage activate carbon(P <0.01),expect D and E group(P >0.05).The best dosage of activate carbon column was 150 g × 3.To make sure the dosage provided theoretics basis for therapy the liver cancer by hemoperfusion.