析因设计筛选异烟肼利福平合用致小鼠肝损害的实验方案

Selection the test method for hepatotoxicity induced by isoniazid and rifampicin in combination by factorial experimental design

目的利用析因设计,筛选利福平(RFP)与异烟肼(INH)合用致小鼠肝损伤的最佳实验方法。方法采用不同的时间(A)、顺序(B)、剂量(C)给药,分别测定ALT的活性,肝匀浆中GSH及MDA的含量。结果影响两药合用致小鼠肝损害程度的因素依次为:给药剂量、时间和顺序(C>A>B);最佳给药方法为同时给药10天,RFP与INH均为100mg·Kg^(-1)·d^(-1)。结论应用析因设计筛选异烟肼利福平合用致小鼠肝损害的实验方案,能较全面地反映各因素水平对实验的影响,方法可行。

OBJECTIVE To select the best test method for hepatotoxicity induced by isoniazid(INH) plus rifampicin(RFP) on mice using factorial experimental design.METHOD INH and RFP were given in different times,sequence,dosages.The serum level of ALT,the hepatic contents of MDA and GSH were measured.RESULTS The factors of influencing the degree of hepatotoxicity on mice in proper order are:dosages,times,sequence.The best experimental method is giving RFP and INH at the same time,for 10 days,RFP and INH are all 100mg·kg^(-1)·d^(-1).CONCLUSION Appling factorial design to select the best test method can overall reflect the factors and levels which influence the indexes of the test,the factorial experimental design is feasible.