连夏消痞胶囊的薄层色谱法鉴别及高效液相法含量测定

Study on Quality Standards for Lian Xia Xiao Pi Capsule

目的制定连夏消痞胶囊的质量标准。方法用薄层色谱法鉴别黄连、生晒参、黄芩。用HPLC测定连夏消痞胶囊中盐酸小檗碱的含量,采用Zorbax SB-C18色谱柱（250mm×4.6mm,5μm）,流动相:乙腈-磷酸盐缓冲液（30:70,pH 3.0）,检测波长345nm,流速为1.0mL/min,柱温为室温。结果①供试品中黄连、生晒参、黄芩有效主成份与其相应的对照药材和对照标准品在薄层色谱上有相同斑点;②盐酸小檗碱范围在0.020.4μg内呈良好的线性关系（r=0.9998）,平均回收率97.87%,RSD为1.68%。结论其定性定量方法简便、准确、专属性强、重现性好,所建立的方法可供制定连夏消痞胶囊质量标准使用。

OBJECTIVE To build up quality standards for Lian Xia Xiao Pi capsules.METHOD The qualitative analyses were carried out with Rhizoma coptidis,Radix ginseng and Radix Scutellariae by TLC and qualitative analyses with Hydrochloride Berberine as an index by HPLC in LianXia Xiao Pi capsules.The HPLC system consisted of Zorbax SB-C_(18) column(250mm×4.6mm,5μm).Acetonitrile-phosphoric acid buffer solution(30:70,pH 3.0) mixture as mobile phase,with detection at 345 nm.Flow rate 1.0mL/min and column temperature at room temperature.RESULTS The Linear range was 0.02~0.4p,g(r= 0.9998).CONCLUSION The results indicated that methods were simple and Sensitive with a good concentration.This Study provided a quality control method for Lian Xia Xiao Pi capsules.