天然没药与胶质没药HPLC特征图谱研究

Study on HPLC Characteristic Chromatograms of Myrrh and Gum opoponax

目的建立天然没药与胶质没药高效液相色谱特征图谱鉴别方法。方法采用Eclipse Plus C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,流速1.0mL·min^(-1),检测波长240 nm,建立天然没药与胶质没药的特征图谱,统计分析比较2种没药的差别。结果建立了天然没药特征图谱,标定7个特征峰;建立了胶质没药特征图谱,标定2个特征峰。2种没药的成分存在明显差异。结论所建立的2种没药的高效液相色谱特征图谱重复性好,专属性强,可用于2种没药的鉴别。

OBJECTIVE To establish HPLC characteristic chromatograms of Myrrh and Gum opoponax. METHODS The characteristic chromatograms of Myrrh and Gum opoponax were established by HPLC method. The separation was performed using Eclipse Plus C18(250 mm×4.6 mm, 5μm) column by gradient elution with acetonitrile and 0.1% phosphoric acid aqueous solution as the mobile phase at a flow rate of 1.0 mL·min-1. The UV detection wavelength was set at 240 nm. Statistical analysis was applied to evaluate the difference between Myrrh and Gum opoponax. RESULTS The HPLC characteristic chromatograms of Myrrh and Gum opoponax were established, in which seven and two characteristic peaks were assigned, respectively. There were significant differences between Myrrh and Gum opoponax. CONCLUSION The established HPLC characteristic chromatograms of Myrrh and Gum opoponax showed good reproducibility and specificity, and can be used for the identification of Myrrh and Gum opoponax.