一测多评法同时测定正天丸中6种指标性成分

Determination of Six Index Components in Zhengtian Pills by Quantitative Analysis of Multi-components by Single Marker

目的建立一测多评(quantitative analysis of multi-components by single-marker,QAMS)法同时测定正天丸中6种指标性成分的含量。方法采用Agilent XDB C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速1.0 mL·min^(-1),检测波长310 nm,柱温30℃,进样量10μL。应用QAMS法,以芍药苷为参照物,测定6种指标性成分之间的相对校正因子,分别采用外标法和QAMS法测定正天丸中的6种指标性成分的含量,并对2种方法计算结果进行对比分析,以验证QAMS法的实用性与稳定性。结果建立了用于测定正天丸中6种指标性成分的QAMS法,并对6批正天丸进行测定,其计算值与测定值的差异较小。结论本研究建立的QAMS法可测定正天丸中6种指标性成分的含量,方法简单、有效,结果准确,可用于正天丸的质量控制,为完善质量标准提供参考。

OBJECTIVE To develop a method of quantitative analysis of multi-components by single marker(QAMS) for six index components in Zhengtian pills. METHODS The method with a Agilent XDB C18 column(250 mm×4.6 mm, 5 μm) was applied, the mobile phase was acetonitrile-0.1% phosphoric acid of gradient elution, the flow rate was 1.0 mL·min-1,the detection wavelength was 310 nm,the column temperature was 30 and the injection volume was 10 ℃μL. Used paeoniflorin as a reference, the relative correction factors among the six kinds index components were detected by QAMS. These six index contents were determined by external standard method and QAMS. The results obtained from two different methods were compared. Verified the practicability and stability of QAMS. RESULTS The established QAMS was used to determine the six index components in Zhengtian pills, and totally six batches of Zhengtian pills were determined. There was no significant difference in the calculated values and the measured values. CONCLUSION The QAMS method is simple, effective and accurate in determining the contents of six index components in Zhengtian pills, which can be used for the quality control of Zhengtian pills and provide reference for the improvement of quality standards.